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KMID : 1159320150170020151
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Korean Journal of Vision Science
2015 Volume.17 No. 2 p.151 ~ p.162
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Identification and gyrA Gene Analysis of Fluoroquinolone Antibiotic Resistant Strains Isolated from Infectious Keratitis Suspected Patient
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Na Hun-Taek
Han Deok-Kyu
Kim Hong-Seon
Kim Soo-Young
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Abstract
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Purpose: As a side effect contact lenses identified the isolates from patients who are suspected to be infectious keratitis, by differentiating a fluoroquinolone antibiotic resistant strains analyzed the gyrA genes involved in resistance mechanisms to investigate the cause of the resistance.
Methods: Targeting the stromal invasion 30 patients suspected infectious keratitis, isolates from the cornea were cultured in Ofloxacin(5㎍/㎖), Ciprofloxacin(5㎍/㎖), Levofloxacin(5㎍/㎖) antibiotic medium was identified by 16S rRNA gene PCR method. Differentiating the resistant strains by the disk diffusion method, and to extract genomic DNA and then amplified through the PCR, analyzed genes gyrA QRDR region.
Results: Strains cultured on the medium supplemented with Ofloxacin were identified as Achromobacter xylosoxidans(N101, N102), Staphylococcus epidermidis(N105), and strains cultured in the medium supplemented with Ciprofloxacin were identified as Achromobacter xylosoxidans(N101, N102), Staphylococcus epidermidis(N103, N104, N105, N106), Staphylococcus warneri(N107), Staphlococcus hominis(N108), Staphylococcus saprophyticus(N109), and Alcaligenes sp.(N110). Staphylococcus epidermidis(N105) was resistant to Ofloxacin, Ciprofloxacin and Levofloxacin was shown to intermediate resistant, the amino acid mutation observed by Ser(TCT)84→Phe (TTT) the analysis results QRDR region of gyrA gene.
Conclusions: In corneal of infectious keratitis suspected patients, seven strains of gram-positive and three strains of gram-negative were identified. observed resistant strain in the antimicrobial susceptibility test, in codon 84 region of the gyrA gene was confirmed that affects the resistance appeared that amino acid mutations. the future, predicted would occur many resistant strains to continued use of antibiotic, will be needed attention to resistance when using a fluoroquinolone antibiotic.
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KEYWORD
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Infectious keratitis, fluoroquinolone, resistance, gyrA
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